EVOLVABILITY AND HIERARCHY IN REWIRED BACTERIAL GENE NETWORKS PDF

Sequencing DNA from several organisms has revealed that duplication and drift of existing genes have primarily molded the contents of a given genome. Though the effect of knocking out or over-expressing a particular gene has been studied in many organisms, no study has systematically explored the effect of adding new links in a biological network. Most importantly, we find that certain networks consistently survive over the wild-type under various selection pressures. Therefore new links in the network are rarely a barrier for evolution and can even confer a fitness advantage. Lower-tier nodes are more sparsely connected and the network structure has a scale-free power-law degree distribution 8 , 9. It has been argued that such networks are particularly robust to random errors since only a few nodes are highly-connected hubs, whose perturbation would affect the network drastically

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In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. A Nature Research Journal. Sequencing DNA from several organisms has revealed that duplication and drift of existing genes have primarily moulded the contents of a given genome.

Though the effect of knocking out or overexpressing a particular gene has been studied in many organisms, no study has systematically explored the effect of adding new links in a biological network. Most importantly, we find that certain networks consistently survive over the wild type under various selection pressures.

Therefore new links in the network are rarely a barrier for evolution and can even confer a fitness advantage. Blattner, F.

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Evolvability and Hierarchy in Rewired Bacterial Gene Networks

Sequencing DNA from several organisms has revealed that duplication and drift of existing genes have primarily moulded the contents of a given genome. Though the effect of knocking out or overexpressing a particular gene has been studied in many organisms, no study has systematically explored the effect of adding new links in a biological network. To explore network evolvability, we constructed recombinations of promoters including regulatory regions with different transcription or a-factor genes in Escherichia coli, added over a wild-type genetic background. Most importantly, we find that certain networks consistently survive over the wild type under various selection pressures. Therefore new links in the network are rarely a barrier for evolution and can even confer a fitness advantage. The E. Lower-tier nodes are more sparsely connected and the network structure has a scale-free power-law degree distribution 8,9.

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Evolvability and hierarchy in rewired bacterial gene networks

Sequencing DNA from several organisms has revealed that duplication and drift of existing genes have primarily moulded the contents of a given genome. Though the effect of knocking out or overexpressing a particular gene has been studied in many organisms, no study has systematically explored the effect of adding new links in a biological network. To explore network evolvability, we constructed recombinations of promoters including regulatory regions with different transcription or sigma-factor genes in Escherichia coli, added over a wild-type genetic background. Most importantly, we find that certain networks consistently survive over the wild type under various selection pressures. Therefore new links in the network are rarely a barrier for evolution and can even confer a fitness advantage. This site needs JavaScript to work properly. Please enable it to take advantage of the complete set of features!

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By adding new connections between unrelated genes to a gene network, researchers can investigate network robustness and evolvability. When researchers want to understand how a biological network functions, typically they overexpress or knock out the gene of interest and watch the effects unfold. Such manipulations can be enlightening for understanding network robustness, which is essential for evolution. Mark Isalan and his colleagues at the Center for Genomic Regulation in Barcelona, Spain have come up with a new approach to test the robustness and evolvability of gene networks by systematically examining the effects of adding new links between unrelated genes in Escherichia coli. As gene duplication is a major driving force for evolution, the researchers tested to see what would happen if open reading frames ORF were duplicated and linked to new promoters. Isalan and colleagues generated such 'rewired' gene networks from the genes for seven master transcription factors, seven sigma factors and eight downstream transcription factors. They created new promoter—ORF fusions, introduced them to the bacteria one at a time, either on plasmids or as E.

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