IMVIC TEST PROCEDURE PDF

An agar plate method was developed for the performance of the IMVic indole, methyl red, Voges-Proskauer, and citrate tests in lieu of the conventional tubed liquid media. By modifying the composition of the media and adding agar, a single "X"-compartmented petri dish was prepared containing all four IMVic test media. Ease of performance and simplification of the test were achieved by inoculating all four media simultaneously from a single colony single inoculum on eosin-methylene blue agar. Tests with 87 cultures, representing 7 genera in the family Enterobacteriaceae, were completed with typical correct IMVic patterns for all cultures within 48 h. Parallel tests with conventional media showed that the agar plate method was superior, more sensitive, faster, and simpler to perform, and less time was required to identify Escherichia coli by 72 h. National Center for Biotechnology Information , U.

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The IMViC tests are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group. A coliform is a gram negative , aerobic, or facultative anaerobic rod, which produces gas from lactose within 48 hours. The presence of some coliforms indicate fecal contamination.

The term "IMViC" is an acronym for each of these tests. The lower case "i" is merely for "in" as the Citrate test requires coliform samples to be placed "in Citrate".

These tests are useful in distinguishing members of Enterobacteriaceae. In this test, the organism under consideration is grown in peptone water broth. It contains tryptophan , which under the action of enzyme tryptophanase is converted to an Indole molecule, pyruvate and ammonium. The indole is then extracted from the broth by means of xylene.

To test the broth for indole production, Kovac's reagent. Kovac's reagent consist of amyl alcohol and para-dimethylaminobenzaldehyde and concentrated hydrochloric acid. Kovac's reagent is actually used to determine ability of an organism to separate indole from amino acid tryptophan and it is added after incubation.

These tests both use the same broth for bacterial growth. The broth is called MRVP broth. After growth, the broth is separated into two different tubes, one for the methyl red MR test and one for the Voges-Proskauer VP test. The methyl red test detects production of acids formed during metabolism using mixed acid fermentation pathway using pyruvate as a substrate. The pH indicator Methyl Red is added to one tube and a red color appears at pH's lower than 4. The VP test uses alpha-naphthol and potassium hydroxide to test for the presence of acetylmethylcarbinol acetoin , an intermediate of the 2,3-butanediol fermentation pathway.

After adding both reagents, the tube is shaken vigorously then allowed to sit for minutes. A pinkish-red color indicates a positive test, meaning the 2,3-butanediol fermentation pathway is used. This test uses Simmon's citrate agar to determine the ability of a microorganism to use citrate as its sole carbon source.

The agar contains citrate and ammonium ions nitrogen source and bromothymol blue as an indicator. The citrate agar is green before inoculation, and turns blue as a positive test indicator, meaning citrate is utilized.

These IMViC tests are useful for differentiating the family Enterobacteriaceae , especially when used alongside the Urease test. The IMViC results of some important species are shown below.

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Archived from the original on Retrieved CS1 maint: archived copy as title link. Clinical microbiology techniques. Asepsis Streak plate Selective media. Bacterial culture Fungal culture Viral culture. Categories : Microbiology.

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Shigella spp. Proteus vulgaris [3]. Citrobacter freundii. Enterobacter aerogenes. Isolation techniques Asepsis Streak plate Selective media.

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IMViC Tests: Principle, Procedure and results

IMViC is an acronym that stands for four different tests. To obtain the results of these four tests, three test tubes are inoculated: tryptone broth indole test , methyl red — Voges Proskauer broth MR-VP broth , and citrate. It is performed on sulfide-indole-motility SIM medium or in tryptophan broth, or in motility urease indole MIU medium. I am Tankeshwar Acharya.

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37: IMViC tests

The IMViC tests are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group. A coliform is a gram negative , aerobic, or facultative anaerobic rod, which produces gas from lactose within 48 hours. The presence of some coliforms indicate fecal contamination. The term "IMViC" is an acronym for each of these tests. The lower case "i" is merely for "in" as the Citrate test requires coliform samples to be placed "in Citrate". These tests are useful in distinguishing members of Enterobacteriaceae. In this test, the organism under consideration is grown in peptone water broth.

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US4187351A - IMViC test method - Google Patents

The invention described herein may be manufactured, used and licensed by or for the Government for governmental purposes without the payment to me of any royalties thereon. The invention relates to a method for conducting bacterial IMViC tests. More particularly, the invention relates to an improved method suitable for conducting multiple bacterial IMViC tests on solid media. Many isolation methods and identifying tests have been developed to determine the identity of bacterial strains. The isolation methods are designed to take advantage of the different growth requirements of the various families of bacteria.

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